The Translational Organ-on-chip Platform (TOP) aims to bridge the gap between organ-on-chip developers and end-users by connecting microfluidic building blocks, such as organs-on-chips and sensors, on fluidic circuit boards in an openly standardized manner. The use of a common, standardized platform facilitates collaboration and chip transfer between partners from different labs to achieve higher level (multi-) organ-on-chip systems. The TOP can be used for a wide range of applications, such as highly parallelized cell culturing, automated medium recirculation and online sensing.