Automated and Multiplexed Organs-on-Chips
Automated, multiplexed cell culturing chips with high spatiotemporal resolution are essential for multiparameter screening and studying response kinetics. Here at AST we are developing highly multiplexed microfluidic chips with integrated valves for programmable cell culture medium exchange and stimulation factor exposure.
Open-TOP (Translational Organ-on-Chip Platform)
Photoresponsive Hybrid PDMS-Hydrogel Microfluidic Valving
Develop hybrid PDMS-hydrogel valves (PHVs) using PDMS membranes and photoresponsive hydrogel materials for organ-on-chip applications. A new, orthogonal microfluidic actuation system would compliment the already multiplexed TOP platform by allowing users to select any combination of culture chambers for perturbation or variable flow conditions. Through the combination of digital micromirror devices (DMDs) and strategically positioned PHVs, flow conditions could be altered mid-experiment without the need for mechanical valving or physical handling of the system.
Standardized open Modular Approach to Recapitulate Tissues (SMART) Organ-on-Chip
Multiplex micro-engineered patient-specific tissues array for artificial intelligence disease prediction
This project will focus on developing a microfluidic array of micro-engineered heart tissues (µEHT) that combine with artificial intelligence (AI) software, aims to predict disease developments in patients. The microfluidic networks will allow users to control the perfusions of each µEHT. Besides, we will assess the effect of cytokines and different drug compounds on patient-specific µEHT. This platform will revolutionize not only research methods in academia, but also for clinical test in hospitals and drug development in industries.